Pmutin-gfp+
WebpMUTIN4-GFP. Catalog No. PVT11301 Packing 2ug Function / Resistance Amp Screen / Strain / Culture temperature Replicon Copy Promoter Induction Forward primer WebApr 8, 2005 · Plasmid pMUTIN-GFP+ is a cloning vector (based on a ColE1-like replicon) designed for construction and expression of fusion genes encoding hybrid proteins composed of N-terminal part of the desired protein (or. PAP I leader sequence directs the protein to cellular membrane.
Pmutin-gfp+
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WebFeb 25, 2011 · FtsZ was detected by immunofluorescence. EzrA–GFP+ was observed as a complete ring, rather than double dots corresponding to a slice through a ring as seen in (A), due to flattening of cells following lysostaphin digestion of the cell wall. Scale bar = 1 µm. ... The PCR product was digested with KpnI and EagI and inserted into pMUTIN–GFP+ ... Webits promoter from pXX167 (17) into the Clal site of pMUTIN-GFP+ (46). This construction gave rise both to a wild-type SopA-GFP plasmid and to another plasmid that had two spon …
WebMay 28, 1998 · Plasmid pMUTIN-GFP+ is a cloning vector (based on a ColE1-like replicon) designed for construction and expression of fusion genes encoding hybrid proteins composed of N-terminal part of the desired protein (or its part) and green fluorescent protein (GFP) [18]. Show abstract. WebFeb 3, 2011 · For FACS experiments performed 7–14 days after muscle dissociation, cultured nestin-GFP+ cells were washed with phosphate-buffered saline (PBS) and treated with 0.25% trypsin/0.05% EDTA to isolate them in a suspension. When all cells were detached, the enzymatic reaction was stopped with the resuspension medium described …
http://muchong.com/t-5006068-2 WebJan 1, 2016 · Transformants were PCR confirmed using pMUTIN-GFP gene specific primers ( Table S1) that were designed from gfp gene sequences for amplification of 359 bp. PCR was performed in GoTaq PCR kit of Promega (GoTaq Buffer-1X, MgCl 2 – 1.5 mmol l −1, dNTPs – 200 μmol l −1, Forward/Reverse primers – 10 pmol each, DMSO – 6%, Taq …
WebJan 29, 2016 · Two separate plasmids namely pMUTIN-GFP+ and pDG1662 were used for the construction of plasmid pDG1662-A. On isolation of genomic DNA from B. subtilis …
WebThe mechanism of pBacTag Tagging Vectors is illustrated with pBacTag-GFP+ as example. This vector can be used for creating a GFP+ fusion protein from any chromosomally located gene of interest, by fusing a gfp+ tag to the chosen gene. In this example the gene of interest is named orf2. It is part of an operon, including three genes how diwali is celebrated in gujratWebMay 7, 2010 · We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers … how dles deta 9 gummies feelWebThe plasmid, pKW244, carrying the mcpB-gfpmut3 fusion was built in three steps. First, the gfpmut3 gene was amplified by PCR using pGFPmut3 ( 34 ) as the template and inserted into the ClaI and SpeI restriction sites of plasmid pMUTIN-GFP+ ( 35 ), replacing gfp with gfpmut3 and yielding the plasmid pKW235. how dlopen worksWebJun 25, 2015 · Plasmid pMutin-GFP+ contains a gfp reporter with three terminators (t1, t2, t0 from the rrnB operon of E. coli ) upstream the multiple cloning site in front of gfp and a trpA terminator downstream of gfp . pMutin-GFP+ was amplified with primers PG187 and PG188 in order to remove the Pspac promoter and to introduce 5 unique restriction sites in ... how dk you get free muaicWeb你好 师姐 你有pmutin质粒吗？ 师妹最近也在做芽孢的敲除实验 但是一直做了4个月了 都毫无收获 现在已经研三了 可以把你的质粒卖给我吗 谢谢 赞 一下 how dj edit songs liveWebpMUTIN-cMYC - antibody tagging vector inserts into specified chromosomal site, adds antibody tag for protein purification pSG1164/pMUTIN-GFP+ c-terminal GFP fusion plasmid, inducible with xylose/IPTG, insert at any amyE/any point pMUTIN4 aiiA knockout if they have it pDG1728 test promoters need strain 1A771 for amyE recombination with ERM? how dlls workWebDec 1, 1998 · The integration of a pMUTIN vector into the target gene has three consequences: (1) the target gene is inactivated; (2) lacZ becomes transcriptionally fused to the gene, allowing its expression... how dls score is calculated