Web28 apr. 2024 · The biliary system is routinely accessed for clinical purposes via endoscopic retrograde cholangiopancreatography (ERCP). We previously pioneered ERCP-mediated hydrodynamic injection in large animal models as an innovative gene delivery approach for monogenic liver diseases. However, the procedure poses potential safety concerns … Web31 mrt. 2024 · The principle of hydrodynamic delivery was initially used to develop a method for the delivery of plasmids into mouse hepatocytes through tail vein injection and has been expanded for use in the delivery of various biologically active materials to cells in various organs in a variety of animal species through systemic or local injection, …
The Modified One-Dimensional Hydrodynamic Model Based on …
Web21 aug. 2015 · Hydrodynamics-based delivery utilizes hydrodynamic pressure induced by the volume and flow of injection as the driving force to facilitate intracellular gene transfer [1,2].The original method of hydrodynamic gene delivery (HGD) in small animals involves tail vein injection of plasmid DNA solution in a volume equal to 8%–10% of the body … Web22 jun. 2024 · Hydrodynamic force generated by pressurized injection of solution into the blood vessel could breach the endothelium and the closely associated hepatocyte plasma membrane. This approach was successfully used in mice to deliver transposons and CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats–CRISPR … ethernet matrix
Hydrodynamic injection of viral DNA: A mouse model of acute …
WebMoreover new animal models are possible to be created for study human diseases, mainly viral due to the fact that they are species specific 5,8,10. 3. Animal models 3.1 Mice The majority of the research protocols of the hydrodynamic delivery in mice are based on tail vein injection, due to the ease of application and the pos itive results 11,14-53. WebHGD is a method that facilitates the delivery and expression of genetic material in target cells through an intravenous injection. It is developed based on advances in our understanding of the structure and properties of blood capillaries, and the dynamic properties of fluids passing through blood vasculature. Webwater Article The Modified One-Dimensional Hydrodynamic Model Based on the Extended Chezy Formula Junwei Zhou 1,* , Weimin Bao 1, Yu Li 2, Li Cheng 2 and Muxi Bao 3 1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, College of Hydrology and Water Resources, Hohai University, Nanjing 210098, China; … firehouse nursery school