WebIn this application note, we demonstrate the advantages of LC-MS to evaluate the 5’ capping efficiency of synthetic mRNA using an easy-to-use, compliant-ready BioAccord LC-MS System and ACQUITY Premier Oligonucleotide BEH C 18 Columns, which feature MaxPeak High Performance Surfaces Technology. Web29 de jan. de 2024 · RNase H cleavage confirmed high capping efficiencies for enzymatic RNAs previously detected by ribozyme-mediated cleavage (purified hAg GCG: 75–85%). For ARCA samples, rather low capping efficiencies of 37–47% were obtained (Figure 6, Table 5), which is in accordance with the data shown in Section 3.3.
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